Single cell label-free imaging

SID4 Element


ADVANCED ADD-ON | for quantitative phase microscopy

The SID4Element combines a sCMOS camera to provide the most accurate quantitative phase images. It suits large population imaging as it offers a large field of view. The system also enables acquiring phase and fluorescence with the same camera for an easy merging.

  • 10 μm phase pixel : best possible spatial resolution, even at low magnification
  • Large field of view, adjustable with acquisition speed
  • Artefact-free and instantaneous phase image
  • High sensitivity fluorescence with the same camera



Wavelength range400–800 nm
Field of viewUp to 8.53 x 7.20 mm
Image size (phase pixels) Up to 853 x 720 pixels
Lateral resolution (Phase image pixels cell size)10 µm without magnification
Resolution (Phase) < 2 nm RMS
up to 0.1 nm
Acquisition rate Up to 200Hz
Microscope interface C-mount
Camera interfaceC-mount, USB 3.0

These specifications are given for the combination with a sCMOS Zyla 5.5 camera

SID4Bio Software


The SID4Bio software is designed to assist image acquisition and analysis. It delivers a complete set of parameters based on morphometric and phase information contained in the SID4Bio camera image.

high content imaging


Image acquisition
  • Image stack management
  • Time-lapse
  • Compatible with Metamorph or Micromanager
Post-processing features
  • Automated segmentation – adapted to adherent cells, round cells, bacteria, yeast, ...
  • Tracking
  • Thresholding
  • Single cell dry mass and morphometry
  • Scatter plot for population identification
  • Cell counting

For an OEM integration or automated microscopy set-up, the SID4Bio software is compatible with MetaMorph, Micromanager, ImageJ and a SDK toolkit is available

Contact us to get those products