Phase + fluorescence microscopy

Introduction

Phasics offers a unique solution for easily combining fluorescence and quantitative phase microscopy. Thanks to a smart add-on, the same camera captures images from both modalities. So the merging is immediate and the set-up is very simple.
 
Merging both techniques provides a very comprehensive dataset on the specimen for deep understanding of biological events:

  • Quantitative phase imaging offers great contrast for label-free cell imaging and tracking and allows easily measuring cell morphological parameters and cell dry mass
  • Fluorescence microscopy provides highly specific information related to the presence of fluorophores for identification of targeted components at cellular or molecular levels

For

MOLECULAR & PHYSIOLOGICAL DATA
At single cell level


  • Correlate molecular pathways and cellular data to understand complex biological event

 

VALIDATE PHASE MEASUREMENT
Use phase to identify elements


  • Use fluorescence to calibrate phase information

 

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ASSIST FLUORESCENCE IMAGING
Use phase to see and track cells


  • See the environment of the labelled specimens
  • Check fluorescence expression
  • Prevent useless photo-bleaching by targeting cells of interest through artefact-free phase imaging

Introduction

Quantitative phase and fluorescence microscopy merging is easy and fast with Phasics solution. The same camera acquires images from both modalities thanks to a smart add-on, the SID4-Element. This add-on integrates Phasics patented technology. With this solution, merging is immediate and set-up is very simple.

Set Up

Phasics patented technology stands out for its integration as a simple camera at the end of the microscope. Thus it easily combines to other modalities such as fluorescence microscopy. With SID4 Element add-on, switching between fluorescence and phase only requires a deflector
 
Note: this set-up can also apply to other multi-modality experiment

Phase and fluorescence microsocopy-set-up

Advantages

IMMEDIATE MERGING
by using the same camera


  • Quantitative phase image and fluorescence images are acquired with the same camera so they immediatly overlay

SIMPLE SET-UP
with one camera


  • The system plugs on to the microscope through the C-mount port. No change is needed on the microscope
  • Any plate are compatible, including multiwell plates

SIMULTANEOUS INFORMATION
at both cellular and molecular level


  • Switching between modalities is immediate

MULTIMODALITY TIME-LAPSE
Elaborated acquisition schemes


  • Acquisition of Phase and fluorescence images can be planned so as to use phase to track cell and use fluorescence only when needed

Introduction

Combining quantitative phase and fluorescence microscopy finds application in cell biology Research, in pathology study and even in bioproduction

Lysosome identification
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Red blood cell pathophysiology
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Tubulin microtubule + Actin filament
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Cell infection
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Scientific publications

Biophysical Journal, 106,8 pp 1588-1595

DOI: 10.1016/j.bpj.2014.02.023

Fast label-free cytoskeletal network imaging in living mammalian cells

Application Note

Lysosome label-free identification with quantitative phase imaging