Cytology

Introduction

LABEL-FREE CYTOMETRY | with quantitative phase microscopy


Phasics quantitative phase microscopy solution allows analyzing large living cell population at single cell level. Indeed it delivers a comprehensive dataset of accurate quantitative parameters for individual cell such as cell morphology (surface, shape factor…) or cell dry mass and many phase-shift related parameters (density, homogeneity, distribution). Thus it is ideal for multiplex assays as an automated image cytometer.
 

► Artefact-free images ensure robust and automated segmentation and measurements.
► As the technique is label-free imaging technique, it allows long period time lapse microscopy for non-invasive cellular study. It applies to measure cell motility, cell proliferation, monitoring of cell cycle, apoptosis, viability, differentiation, cytotoxicity
Merging with fluorescence is possible to get a comprehensive dataset about the events at cellular and molecular level.

For

  • Cancer Cell proliferation & growth rate
  • Pharmacology Research: Drug screening , Drug discovery , Cytotoxicity assays
  • Bioprocess: Cell culture monitoring, microbiology
  • Blood testing : Red blood cell pathology identification such as Anemia type identification, malaria parasite counting
  • Stem cell monitoring & selection in regenerative medecine

Advantages

HIGH CONTENT IMAGING
Study each single cell of a large population


  • New phase-based parameters (homogeneity, density…) in addition to morphometric information for accurate diagnostic
  • Robust measurement of morphometric parameters
  • Quantitative and statistical study of large cell population based on multiple parameter measurement: dry mass, surface, density…
  • Multi-parametric single cell classification (homogeneity, shape, dry mass, presence of abnormal vesicles…)
    •  
      subpopulation sorting

HIGHLY RELIABLE MEASUREMENT
High accuracy and artefact free


  • Automated segmentation and analysis thanks to reliable phase shift measurement
  • Artefact-free images for robust segmentation and morphometric measurement
  • High sensitivity phase measurement for accurate dry mass estimation
  • Reliable large population statistics
  • Comparable quantitative diagnostic

Label-free-cell-segmentation

LABEL-FREE IMAGING
Non-invasive and fast preparation


  • Fast protocol
  • Time-lapse microscopy over long period of time
  • No photobleaching
  • Cell monitoring over long period of time: cell proliferation, stem cell differentiation, bioproduction
  • Non-invasive cell selection for regenerative medecine

 
Intracellular imaging

EASY INTEGRATION
with any microscope or cytometer


  • Plug & play and compact camera to integrate in larger set-up such as incubator, cytometer, high content screening stations or dedicated diagnostic instruments
  • Compatible with multi-well plates for long duration time lapse imaging
  • Easy merging with other modalities to get even more data: Fluorescence for simultaneous information at both cellular & molecular, Guidance for Raman spectrometry

quantitative phase microscopy camera

Set Up

QUANTITATIVE PHASE IMAGING
with a camera-like instrument


  • Easy set-up
  • Robust segmentation
  • Valuable measurement for each single cell

individual cell dry mass

 

PHASE + FLUORESCENCE MICROSCOPY
with the same camera


  • Immediate merging
  • Both molecular and cellular data for each individual cell

individual cell dry mass

 

Introduction

Dry mass and morphometric parameters are proven indicators of many cell mechanisms: viability (apoptosis detection…), cell growth (cell cycle status, proliferation…), cell differentiation. They also reveal cell abnormalities: shape change, heterogeneity, presence of parasite… They are useful parameters to identify and monitor cells in large population.

Example

CELL GROWTH & APOPTOSE FOR CANCER RESEARCH

Understand cancer cell interactions and proliferation factors

Single cell growth rate
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Cancer cell scratch
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BLOOD TESTING: pathology and disorder identification

Identify red blood cell blood disorder and blood-related pathology

Malaria diagnostic
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Anemia diagnostic
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Reticulocyte classification
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Red blood cell pathophysiology
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DRUG TESTING & TOXICOLOGY

Quantify effects on cell mechanisms

Drug screening
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Cardiac cell
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Cell motility
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Infection
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BIO PRODUCTION & MICROBIOLOGY

Get non-invasive live cell time-lapse for cell culture monitoring and for regerative medicine (Stem cell, cornea cell)

Stem cell differentiation
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Cell culture monitoring
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Bacteria proliferation
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RESEARCH

Observe and measure cell-cell interaction or intracellular mechanisms

Neuron
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Adhesion fiber motility
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Cytoskeleton
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Bacteria proliferation
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Intracellular components
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Scientific publications

J. Biomed. Opt. 20(12), 126009 (Dec 22, 2015)

DOI: 10.1117/1.JBO.20.12.126009

Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion

Applied Physics Letter 106, 233703

DOI: 10.1063/1.4922525

Phase imaging microscopy for the diagnostics of plasma-cell interaction

Press release

"Fifth edition of the FIEEC Applied Research Prize" (French press release)

The 2nd prize was given to Serge Monneret from Institut Carnot STAR to reward his collaboration with Phasics for quantitative phase microscopy"
French press release on FIEEC site

Biophysical Journal, 106,8 pp 1588-1595

DOI: 10.1016/j.bpj.2014.02.023

Fast label-free cytoskeletal network imaging in living mammalian cells

Journal of Biomedical Optics, SPIE

DOI: 10.1117/1.JBO.17.7.076004

Optical detection and measurement of living cell morphometric features with single-shot quantitative phase microscopy

Optics Express, Vol. 17, Issue 15, pp.13080-13094

DOI: 10.1364/OE.17.013080

Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells

Application Note

Cell dry mass to monitor single cell growth

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Red Blood Cell disorders classification using dry mass and morphology

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Malaria parasite counting using fast label-free protocol

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Lysosome label-free identification with quantitative phase imaging

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