Single cell label-free imaging

Introduction

LABEL-FREE CELL IMAGING
with high sensitivity and no artefact


Phasics technique generates artefact-free highly contrasted images without any fluorophore. It is perfectly suited for label-free imaging of cell membrane and organelles such as mitochondria or vesicles.

► The technique is label-free so it enables non-invasive time-lapse microscopy.
► The technique very easily combines to fluorescence microscopy to observed labelled molecules in their environment.
► The technique uses 1 camera acquisition for 1 phase image. It shows no artefact due to motion (vesicles, membranes).

For

  • Cell growth and cell-cell interaction in Neurology
  • Cell polarity and membrane dynamics
  • Intracellular trafficking

Advantages

LABEL-FREE
Non-invasive imaging of intracellular elements


  • Fast protocol
  • Non invasive
  • Time lapse microscopy over long period of time

Label-free-cell-image-COS7-cell

HIGH SENSITIVITY + ARTEFACT-FREE
Label-free membrane imaging


The technique uses a single shot acquisition in white light by a smart camera:

  • No artefact due to motion
  • No artefact due to the introduction of optical element before the sample
  • No noise due to the use of a laser
  • Possibility to easily average acquisitions to reduce noise

MOLECULAR & CELLULAR DATA AT ONCE
Easy phase and fluorescence imaging


  • Correlate molecular pathways and cellular data to understand complex biological event
  • Accurate and instantaneous localization of labelled proteins as phase and fluorescence images are acquired with the same camera

 

Set Up

QUANTITATIVE PHASE IMAGING
with a camera-like instrument


  • Easy set-up
  • Accurate phase shift measurement

Quantitative phase microscope camera

 

PHASE + FLUORESCENCE MICROSCOPY
with the same camera


  • Immediate merging
  • Both molecular and cellular data for each individual cell

 

Achievements

CELL MEMBRANE

Cell membrane dynamics with high sensitivity and accuracy

Cytoskeleton
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Tubulin microtubule + Actin filament
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Adhesion fiber motility
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ORGANELLES

Intracellular mechanisms study

Lysosome identification
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Intracellular content
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Single cell monitoring and selection

Non-invasive and accurate cell imaging

Neuron
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In vitro fecondation
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Scientific publications

Biophysical Journal, 106,8 pp 1588-1595

DOI: 10.1016/j.bpj.2014.02.023

Fast label-free cytoskeletal network imaging in living mammalian cells

Application Note

Lysosome label-free identification with quantitative phase imaging

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