Label-free tissue imaging

Introduction

LABEL-FREE QUANTITATIVE STRUCTURE IDENTIFICATION IN TISSUE
| high-contrast and numerical indication


Phasics solution delivers highly contrasted images of tissues without any staining. These images have no artefact and help observing structures in the tissue such as cells, fibers and vessels composing the extra cellular matrix. They also help identifying tumorous area in the tissue.

 

As the technique is label-free, the protocol is fast and easy. Besides, when coupling Phasics technique to a polarizer, specific contrast enhancement is offered for structures as collagen in tissues or stress fibers in cells…

For

  • Suitable for digital pathology
  • Fundamental mechanism study
  • Fiber detection and identification

Achievements

HIGH CONTRAST IN TISSUE
No label – No staining


  • Label-free imaging
  • Fast protocol
  • Accurate tissue morphology study

Label-free-tissue-imaging

FIBER STRUCTURE IDENTIFICATION
With birefringence imaging


  • Label-free identification of actin
  • Collagen network architecture in tumorous tissue

birefringence-imaging_Collagen-orientation

MEASUREMENTS IN THICK TISSUE
Accurate quantitative data


  • Valuable quantitative parameter measurements thanks to an unique technique for quantitative phase tomography

Introduction

Phasics technique measures the local delay introduced in light path by the tissue. This delay – also called phase shift- is proportional to the local constituent “density”. Thus Phasics technique offers a very high contrast in the tissue without any staining or reagent.

Set Up

For tissue imaging, Phasics instrument plugs on to any microscope as a simple camera.
label-free-tissue-microscopy
 
SET-UP FOR BIREFRINGENCE IMAGING:
For structured elements imaging such as fibers, an additional polarizer is placed at the light source level. By measuring phase shifts for different light polarization directions, Phasics technique obtains local birefringence data. A “retardance” image is obtained for which each pixel is the local retardance introduced by the specimen. In this image, all birefringent structures such as fiber appear with high contrast and can be identified thanks to their pixel values.
Polarization microsocpy

Advantages

LABEL-FREE


  • Fast protocol

ARTEFACT-FREE


  • Reliable observation and measurement

PLUG & PLAY CAMERA


  • Easy-to use
  • Suitable to any microscope

BIREFRINGENCE IMAGING
with only one polarizer


  • Quantitative data for structure identification on the image
  • Simple set-up with a single additional element
  • Suitable to any microscope

Camera-like instrument for quantitative phase imaging

Introduction

LABEL-FREE TISSUE IMAGING

Fast protocol without staining nor labelling

Cancer biopsy
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Tumoral tissue density
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Diatom
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Quantitative phase tomography
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STRUCTURE OBSERVATION

Birefringence imaging

Collagen
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Collagen orientation in tumor
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Scientific publications

Optics Express, Vol. 23, No. 12, pp. 16383-16406

DOI: 10.1364/OE.23.016383

Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry

Opt. Express 22, 8654-8671

DOI: 10.1364/OE.22.008654

Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination

Optics Letters, Vol. 37, No. 10

DOI: 10.1364/OL.37.001631

Tomographic diffractive microscopy with a wavefront sensor

ACS Nano

DOI: 10.1021/nn2047586

Thermal Imaging of Nanostructures by Quantitative Optical Phase Analysis

Physical Letters, American Physical Society

DOI: 10.1103/PhysRevB.86.165417

Quantitative absorption spectroscopy of nano-objects

Optics Letters, Vol. 37, No. 17

DOI: 10.1364/OL.37.003531

Imaging the Gouy phase shift in photonic jets with a wavefront sensor

Physical Review Letters, American Physical Society

DOI: 10.1103/PhysRevLett.109.093902

Wide-Field Vibrational Phase Imaging

Optics Letters, Vol. 37, No. 10

DOI: 10.1364/OL.37.001718

Modelling quantitative phase image formation under tilted illuminations