Histology

Introduction

LABEL-FREE TISSUE IMAGING


High-contrast for tissues

Phasics solution delivers highly contrasted images of tissues without any staining nor reagent. These images have no artefact and help observing structures in the tissue such as cells, fibers and vessels composing the extra-cellular matrix. They also help identifying tumorous area in the tissue. As the technique is label-free, the protocol is fast and easy.

Label-free-tissue-imaging

This high contrast is generated using the local delay introduced in light path by the tissue. This delay – also called phase shift- is proportional to the local constituent “density”.
 
Specific contrast for fibers
When using polarized light, specific contrast enhancement is offered for structures as collagen in tissues or stress fibers in cells…
 
Quantification in thick tissues
Thanks to an advanced phase tomography technique based on an easy z-scan of a sample, valuable quantitative parameter measurements are made in thick tissue.

For

  • Patient clinical follow-up – biopsy, anatomic pathology including digital pathology and rapid intraoperative examinations
  • Cancer diagnostics and monitoring using collagen orientation in tumor
  • Fiber detection and identification
  • Tissue morphology study

Achievements

LABEL-FREE & STAIN-FREE
No label – No staining


  • Non-invasive
  • Fast protocol: no labelling, no staining – possible use in intraoperative examination
  • Accurate tissue morphology study

Label-free-tissue-imaging

SPECIFIC CONTRAST FOR FIBERS
Easy birefringence imaging


  • Label-free identification of fibers
  • Collagen network architecture in tumorous tissue
  • Easy solution with only one polarizer, suitable to any microscope

birefringence-imaging_Collagen-orientation

EASY SET-UP
Simple camera-like instrument


  • Plug & Play system on to any commercial microscope
  • Working with white light

MEASUREMENTS IN THICK TISSUE
Accurate quantitative data


  • Valuable quantitative parameter measurements thanks to an unique technique for quantitative phase tomography
  • without any staining nor reagent

ARTEFACT-FREE


  • Reliable observation and measurement

Set Up

QUANTITATIVE PHASE CONTRAST IN TISSUE

with a camera-like instrument


  • Easy set-up
  • Artefact-free
  • Label-free

Quantitative phase microscope camera

BIREFRINGENCE IMAGING

With a very easy set-up


  • Combination of a camera-like instrument and only one polarizer
  • Label-free fast protocol
  • Quantitative information

3D TISSUE IMAGING

Only one Z scan for tomographic images


  • Only one camera-like instrument and a motorized stage
  • Label-free fast protocol
  • Quantitative information

Phase Tomography

Example

Phasics innovative technique enables fast protocols for tissue imaging with no staining nor labelling. The resulting images are artefact-free and offers high contrast to observe tissue structures including cell and fibers.

LABEL-FREE TISSUE IMAGING

Fast protocol without staining nor labelling

Cancer biopsy
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Tumoral tissue density
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STRUCTURE OBSERVATION

Birefringence imaging

Collagen
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Collagen orientation in tumor
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Actin stress fiber
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QUANTITATIVE PHASE TOMOGRAPHY

Measurement through thick tissues and large specimens

Diatom
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Quantitative phase tomography
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Scientific publications

Optics Express, Vol. 23, No. 12, pp. 16383-16406

DOI: 10.1364/OE.23.016383

Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry

Opt. Express 22, 8654-8671

DOI: 10.1364/OE.22.008654

Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination

Optics Letters, Vol. 37, No. 10

DOI: 10.1364/OL.37.001631

Tomographic diffractive microscopy with a wavefront sensor

ACS Nano

DOI: 10.1021/nn2047586

Thermal Imaging of Nanostructures by Quantitative Optical Phase Analysis

Physical Letters, American Physical Society

DOI: 10.1103/PhysRevB.86.165417

Quantitative absorption spectroscopy of nano-objects

Optics Letters, Vol. 37, No. 17

DOI: 10.1364/OL.37.003531

Imaging the Gouy phase shift in photonic jets with a wavefront sensor

Physical Review Letters, American Physical Society

DOI: 10.1103/PhysRevLett.109.093902

Wide-Field Vibrational Phase Imaging

Optics Letters, Vol. 37, No. 10

DOI: 10.1364/OL.37.001718

Modelling quantitative phase image formation under tilted illuminations